Foregene factory supply Animal Tissue Direct PCR kit for research use and large quantity of animal tissues

Описание и отзывы

Характеристики

Description:

This kit uses a unique lysis buffer system to quickly release genomic DNA from animal tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.

2× PCR Easy^TM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, reaction buffer, PCR optimizer and stabilizer.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Specifications:

50×20µl rxns,200×20µl rxns,500×20µl rxns,2000×20µl rxns

Kit components:

Features&advantages:

-No time-consuming and expensive DNA purification

-The sample demand is small, as little as 5mg animal tissue can be tested.

-No special treatment such as grinding and crushing is required, and the operation is simple.

-Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

Kit parameters:

-Application: a variety of animal tissues.

-DNA released from sample lysis: only used as a PCR template.

-The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

Work Flow: 

Storage and Shelf life:

Part I of this kit should be stored at 2-8℃.

The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃  for longer storage.

- Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.

-Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.

Part II of this kit should be stored at -20℃ .

-Reagent 2×PCR Easy^TM Mix can be stored for 12 months at -20℃ ; if it is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

Foregene Co., Ltd is specialtized in the IVD kits and R&D reagents production and services, which is integrated in R&D, production and marketing. Foregene has the world’s leading Direct PCR platform.The main products include various IVD kits, nucleic acid purification reagents,PCR and Direct PCR reagents,Genotyping kits, etc. The technology and product performance indicators used are all leading the world status,widely applied in hospitals, institutions, medical laboratories, etc.

   Foregene Co., Ltd. was founded in April 2011 by well-known scholars who returned from studying at Yale University, famous inventors and senior industry professionals.Foregene’s R&D team consists of 40 people(mainly phD and master), has 2500 m² comprehensive building, 600 m² P2 laboratory, and 1200m² GMP production facility.

With Direct PCR technology, there’s no need for cumbersome nucleic acid purification, the sample is directly added to the system and then on the machine.Now all indicators of Foregene’s Direct PCR technology meet the needs of clinical diagnosis.